The Netherlands international picked up the injury during training for this weekend's La Liga fixture against Villarreal. Initial tests suggest a muscle tear that could keep him sidelined until early April.
De Jong's absence comes at a difficult time for Barcelona, who had just welcomed back Pedri from injury. Manager Hansi Flick will need to adjust his midfield options with players like Marc Bernal, Marc Casado, or Eric García likely to fill the gap.